Registration began Monday morning for the second annual M-22 Challenge Triathlon, which will be held June 12 in the Glen Lake area and be limited to 300 participants.

Organizers of the M-22 Challenge also announced they are partnering with the Leland Wine & Food Festival, which will also be held June 12.

The triathlon involves having participants bike around Glen Lake, then heading onto little Glen Lake to race in a “paddle-propelled water craft” before completing the third and final leg, a three-mile run that includes the Dune Climb at the Sleeping Bear Dunes National Lakeshore.

The bike race will start the triathlon in Glen Arbor, a change from the inaugural event when cyclists began from the parking lot of Little Glen Lake Park.

All participants must register online at www.m22challenge.com. A portion of proceeds will benefit the Leelanau Conservancy. Susan Wilcox Olson of Suttons Bay, who is helping promote the M-22 Challenge, said 100 of the 300 available spots were taken by Tuesday morning this week.

After the completion of the triathlon, a racers’ party has been scheduled 15 miles up M-22 from Glen Arbor at the Leland Wine & Food Festival.

The event was begun last year by brothers Matt and Keegan Myers, who also run the M-22 apparel business that utilizes a logo of the road sign of the state highway on its T-shirts, hoodies and stickers.

In 2009, Keri Pawielski of St. Joseph, Mich., was the women’s champion in the M-22 Challenge and Jonathan Kuck of Champaign, Ill., took the men’s title. Kuck, whose family has a summer home in Leelanau County, just made headlines by being a member of the U.S. men’s speed skating pursuit team that won a silver medal at the Vancouver Olympics. He also finished eighth in the men’s 10,000 meter race.